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KMID : 1094720070120030282
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 3 p.282 ~ p.288
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Generation of a naive/synthetic antibody specific to botulinum neurotoxin via motif-grafting
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Lee Ji-Chul
Park Sun-Young
Choi Cha-Yong
Chung Jun-Ho
Lee Myung-Shin
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Abstract
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In this study, we describe a new approach to the production of naive/synthetic human antibodies against the botulinum neurotoxin (BoNT). First, peptides that bind to BoNT serotype A (BoNT/A) were screened from a phage display of a combinatorial peptide library. One peptide, designated ANT 12-2 (TLPSPLALLTVH), was determined to interact with BoNT/A, as well as with other serotypes of BoNT. This peptide specifically reacted with the native form of BoNT/A, but not with its formalin-inactivated form. Next, a hybrid naive/synthetic human Fab library was generated via the grafting of a peptide motif from ANT 12-2 into HCDR3 with randomized flanking residues. Through biopanning, the Fab clone, ANTHU-1, which harbors the HCDR3 sequence of VRIQRSPLALLSWGDV, was selected and confirmed in order to retain the same BoNT-binding characteristics as ANT 12-2.
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KEYWORD
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botulinum neurotoxin, antibody, phage display, peptide library
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